Structure of VAT, a CDC48/P97 Atpase Homologue from the Archaeon Thermoplasma Acidophilum as Studied by Electron Tomography

Author(s): Rockel, Beate, Jochen Walz, Reiner Hegerl, Jürgen Peters, Dieter Typke, Wolfgang Baumeister

Journal: FEBS Letters (1999) 451(1): 27-32.

Abstract:
Valosine-containing protein-like ATPase from Thermoplasma acidophilum is a member of the superfamily of ATPases associated with a diversity of cellular activities and is closely related to CDC48 from yeast and p97 from higher eukaryotes and more distantly to N-ethylmaleimide-sensitive fusion protein. We have used electron tomography to obtain low-resolution (2-2.5 nm) three-dimensional maps of both the whole 500 kDa complex and the N-terminally truncated valosine-containing protein-like ATPase from T. acidophilum complex lacking the putative substrate binding domain.