Miniaturized Multichannel Electrospray Ionization Emitters on Poly(Dimethylsiloxane) Microfluidic Devices

Author(s): Kim, Jin-Sung, Daniel P. Knapp

Journal: Electrophoresis (2001) 22: 3993-3999.

Abstract:
A multichannel electrospray ionization (ESI) emitter was fabricated as part of a poly(-dimethylsiloxane) (PDMS) microfluidic device using a three-layer photoresist process which also produces a self-alignment system to make a bonding between the top and bottom PDMS parts. The prototype device (2 cm high x 5 cm wide x 5 cm long) had 16-channels (30 µm wide x 50 µm deep) with emitters of 1 mm length and 60º point angle. The PDMS emitter tips enabled interfacing the device to ESI-mass spectrometry; a stable electrospray from the tips was performed with limits of detection under 1 µM for reference peptides (adrenocorticotropic hormone fragment 1–17, angiotensin I and III).