Avidin-Biotin Micropatterning Methods for Biosensor Applications

Author(s): Orth, Reid N., T. G. Clark, H. G. Craighead

Journal: Biomed. Microdevices (2003) 5(1): 29-30.

Abstract:
High resolution patterning methods have been developed to immobilize functional proteins onto a silicon dioxide surface for biosensor applications. Antibody lines, as small as 5 µm in width, with intervening 5 µm spacings, were patterened on oxidized silicon wafers using avidin-biotin chemistry. The N-hydroxysuccinimide (NHS) ester of photoactivatable biotin was covalently bound to a self assembled monolayer (SAM) of 3-amino-propyltriethoxysilane (3-APTS) after irradiation by 350 nm ultraviolet (UV) light from a 25 W Hg arc lamp. The patterned layers were evaluated using fluorescent imaging of Alexa-488 conjugated without exposure to harmful UV light, extreme pH, toxic chemicals, or high salinity.