Patterned Protein Microarrays for Bacterial Detection

Author(s): Howell, Stephen W., Halina D. Inerowicz, Fred E. Regnier, Ron Reifenberger

Journal: Langmuir (2003) 19(2): 436-439.

Abstract:
Patterned microarrays of antibodies were fabricated and tested for their ability to bind targeted bacteria. These arrays were used in a series of bacterial immunoassays to detect E. coli 0157:H7 and Renibacterium Salmoninarum (RS). Microarrays were fabricated using microcontact printing (µCP) and characterized using scanning probe microscopy (SPM). The high-resolution SPM imaging showed that targeted bacteria had a higher binding selectivity to complementary antibody patterns than to unfunctionalized regions of the substrate. Additional studies indicated a significant reduction in binding of bacteria when the microarrays were exposed to non-specific bacteria. These studies demonstrate how protein microarrays could be developed into useful platforms for sensing microorganisms.