Automated Lifetime Analysis of a Single Yeast Cell

Author(s): Koschwanez, John, Mark Holl, Robert Carlson, Michael McMurray, Daniel Gottschling, Deirdre Meldrum

Journal: Proceedings of the 2005 IEEE (2005) International Conference on Automation Science and Engineering: 13-18.

Abstract:
Yeast pedigree analysis requires a biologist to manually manipulate single yeast cells every 90 minutes for as long as 150 hours. Automating this analysis will allow the rapid completion of experiments that would have previously taken a biologist’s lifetime. In this paper, progress toward automating yeast pedigree analysis is presented. Also presented are two novel and useful tools for biotechnology: a procedure to pattern a ferromagnetic alloy on PDMS, and a method of magnetically capturing a single cell in a microfluidic channel.